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Preliminary Data of a Quantitative Point of Care Test for SARS-CoV-2 Antibodies From Greece.

Identifieur interne : 000007 ( Main/Exploration ); précédent : 000006; suivant : 000008

Preliminary Data of a Quantitative Point of Care Test for SARS-CoV-2 Antibodies From Greece.

Auteurs : Paraskevi C. Fragkou [Grèce] ; Vassiliki Papaevangelou [Grèce] ; Anastasia Antoniadou [Grèce] ; Dimitra Kavvatha [Grèce] ; Agapi Ploussi [Grèce] ; Nikos Pantazis [Grèce] ; Tamta Sirmpilantze [Grèce] ; Christos Psarrakis [Grèce] ; Spyridon A. Pournaras [Grèce] ; Sotirios Tsiodras [Grèce] ; Alexis Kelekis [Grèce]

Source :

RBID : pubmed:32871850

Descripteurs français

English descriptors

Abstract

BACKGROUND

Antibody testing is necessary to identify immune individuals in the post-initial wave of the COVID-19 pandemic.

PATIENTS AND METHODS

We prospectively evaluated the performance of a quantitative point-of-care test (POCT) for SARS-CoV-2 antibodies. The patient group (PG) comprised of hospitalized confirmed COVID-19 cases. Asymptomatic healthcare volunteers with negative rRT-PCR were included in the control group (CG). Measurement of IgM and IgG was obtained by dry fluorescence immunoassay.

RESULTS

Twenty-six PG (65.9±15.4 years old, male 57.7%) and 18 CG (45.6±10.1 years old, male 33.3%) were included. By manufacturer's cut-off (≥0.04 mIU/ml), sensitivity and specificity were 73.08% and 88.89% for IgM and 88.46% and 33.33% for IgG, respectively. Estimated areas under the ROC curve were 0.907 and 0.848 for IgM and IgG, respectively. Results were improved using a cut-off of IgM ≥0.05 mIU/ml and IgG ≥0.10 mIU/ml.

CONCLUSION

Using stringent cut-off values, SARS-CoV-2 antibody POCT detects immune people and can be used during socioeconomic normalization of communities.


DOI: 10.21873/invivo.12138
PubMed: 32871850


Affiliations:


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Le document en format XML

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<term>Adulte d'âge moyen (MeSH)</term>
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<term>Pneumopathie virale (épidémiologie)</term>
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<term>Pneumopathie virale</term>
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<term>Aged</term>
<term>Humans</term>
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<front>
<div type="abstract" xml:lang="en">
<p>
<b>BACKGROUND</b>
</p>
<p>Antibody testing is necessary to identify immune individuals in the post-initial wave of the COVID-19 pandemic.</p>
</div>
<div type="abstract" xml:lang="en">
<p>
<b>PATIENTS AND METHODS</b>
</p>
<p>We prospectively evaluated the performance of a quantitative point-of-care test (POCT) for SARS-CoV-2 antibodies. The patient group (PG) comprised of hospitalized confirmed COVID-19 cases. Asymptomatic healthcare volunteers with negative rRT-PCR were included in the control group (CG). Measurement of IgM and IgG was obtained by dry fluorescence immunoassay.</p>
</div>
<div type="abstract" xml:lang="en">
<p>
<b>RESULTS</b>
</p>
<p>Twenty-six PG (65.9±15.4 years old, male 57.7%) and 18 CG (45.6±10.1 years old, male 33.3%) were included. By manufacturer's cut-off (≥0.04 mIU/ml), sensitivity and specificity were 73.08% and 88.89% for IgM and 88.46% and 33.33% for IgG, respectively. Estimated areas under the ROC curve were 0.907 and 0.848 for IgM and IgG, respectively. Results were improved using a cut-off of IgM ≥0.05 mIU/ml and IgG ≥0.10 mIU/ml.</p>
</div>
<div type="abstract" xml:lang="en">
<p>
<b>CONCLUSION</b>
</p>
<p>Using stringent cut-off values, SARS-CoV-2 antibody POCT detects immune people and can be used during socioeconomic normalization of communities.</p>
</div>
</front>
</TEI>
<pubmed>
<MedlineCitation Status="MEDLINE" Owner="NLM">
<PMID Version="1">32871850</PMID>
<DateCompleted>
<Year>2020</Year>
<Month>09</Month>
<Day>07</Day>
</DateCompleted>
<DateRevised>
<Year>2020</Year>
<Month>09</Month>
<Day>07</Day>
</DateRevised>
<Article PubModel="Print">
<Journal>
<ISSN IssnType="Electronic">1791-7549</ISSN>
<JournalIssue CitedMedium="Internet">
<Volume>34</Volume>
<Issue>5</Issue>
<PubDate>
<MedlineDate>2020 Sep-Oct</MedlineDate>
</PubDate>
</JournalIssue>
<Title>In vivo (Athens, Greece)</Title>
<ISOAbbreviation>In Vivo</ISOAbbreviation>
</Journal>
<ArticleTitle>Preliminary Data of a Quantitative Point of Care Test for SARS-CoV-2 Antibodies From Greece.</ArticleTitle>
<Pagination>
<MedlinePgn>3039-3045</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.21873/invivo.12138</ELocationID>
<Abstract>
<AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Antibody testing is necessary to identify immune individuals in the post-initial wave of the COVID-19 pandemic.</AbstractText>
<AbstractText Label="PATIENTS AND METHODS" NlmCategory="METHODS">We prospectively evaluated the performance of a quantitative point-of-care test (POCT) for SARS-CoV-2 antibodies. The patient group (PG) comprised of hospitalized confirmed COVID-19 cases. Asymptomatic healthcare volunteers with negative rRT-PCR were included in the control group (CG). Measurement of IgM and IgG was obtained by dry fluorescence immunoassay.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">Twenty-six PG (65.9±15.4 years old, male 57.7%) and 18 CG (45.6±10.1 years old, male 33.3%) were included. By manufacturer's cut-off (≥0.04 mIU/ml), sensitivity and specificity were 73.08% and 88.89% for IgM and 88.46% and 33.33% for IgG, respectively. Estimated areas under the ROC curve were 0.907 and 0.848 for IgM and IgG, respectively. Results were improved using a cut-off of IgM ≥0.05 mIU/ml and IgG ≥0.10 mIU/ml.</AbstractText>
<AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">Using stringent cut-off values, SARS-CoV-2 antibody POCT detects immune people and can be used during socioeconomic normalization of communities.</AbstractText>
<CopyrightInformation>Copyright© 2020, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y">
<Author ValidYN="Y">
<LastName>Fragkou</LastName>
<ForeName>Paraskevi C</ForeName>
<Initials>PC</Initials>
<AffiliationInfo>
<Affiliation>Fourth Department of Internal Medicine, Attikon University Hospital, National and Kapodistrian University of Athens, Athens, Greece.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Papaevangelou</LastName>
<ForeName>Vassiliki</ForeName>
<Initials>V</Initials>
<AffiliationInfo>
<Affiliation>Third Department of Pediatrics, Attikon University Hospital, National and Kapodistrian University of Athens, Athens, Greece.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Antoniadou</LastName>
<ForeName>Anastasia</ForeName>
<Initials>A</Initials>
<AffiliationInfo>
<Affiliation>Fourth Department of Internal Medicine, Attikon University Hospital, National and Kapodistrian University of Athens, Athens, Greece.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Kavvatha</LastName>
<ForeName>Dimitra</ForeName>
<Initials>D</Initials>
<AffiliationInfo>
<Affiliation>Fourth Department of Internal Medicine, Attikon University Hospital, National and Kapodistrian University of Athens, Athens, Greece.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Ploussi</LastName>
<ForeName>Agapi</ForeName>
<Initials>A</Initials>
<AffiliationInfo>
<Affiliation>Second Department of Radiology, Attikon University Hospital, National and Kapodistrian University of Athens, Athens, Greece.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Pantazis</LastName>
<ForeName>Nikos</ForeName>
<Initials>N</Initials>
<AffiliationInfo>
<Affiliation>Hygiene, Epidemiology and Medical Statistics, Medical School, National and Kapodistrian University of Athens, Athens, Greece.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Sirmpilantze</LastName>
<ForeName>Tamta</ForeName>
<Initials>T</Initials>
<AffiliationInfo>
<Affiliation>Fourth Department of Internal Medicine, Attikon University Hospital, National and Kapodistrian University of Athens, Athens, Greece.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Psarrakis</LastName>
<ForeName>Christos</ForeName>
<Initials>C</Initials>
<AffiliationInfo>
<Affiliation>Fourth Department of Internal Medicine, Attikon University Hospital, National and Kapodistrian University of Athens, Athens, Greece.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Pournaras</LastName>
<ForeName>Spyridon A</ForeName>
<Initials>SA</Initials>
<AffiliationInfo>
<Affiliation>Clinical Microbiology Laboratory, Attikon University General Hospital, National and Kapodistrian University of Athens, Athens, Greece.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Tsiodras</LastName>
<ForeName>Sotirios</ForeName>
<Initials>S</Initials>
<AffiliationInfo>
<Affiliation>Fourth Department of Internal Medicine, Attikon University Hospital, National and Kapodistrian University of Athens, Athens, Greece.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Kelekis</LastName>
<ForeName>Alexis</ForeName>
<Initials>A</Initials>
<AffiliationInfo>
<Affiliation>Second Department of Radiology, Attikon University Hospital, National and Kapodistrian University of Athens, Athens, Greece akelekis@med.uoa.gr.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList>
<PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo>
<Country>Greece</Country>
<MedlineTA>In Vivo</MedlineTA>
<NlmUniqueID>8806809</NlmUniqueID>
<ISSNLinking>0258-851X</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList>
<Chemical>
<RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D000914">Antibodies, Viral</NameOfSubstance>
</Chemical>
<Chemical>
<RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D007074">Immunoglobulin G</NameOfSubstance>
</Chemical>
</ChemicalList>
<SupplMeshList>
<SupplMeshName Type="Disease" UI="C000657245">COVID-19</SupplMeshName>
<SupplMeshName Type="Organism" UI="C000656484">severe acute respiratory syndrome coronavirus 2</SupplMeshName>
</SupplMeshList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList>
<MeshHeading>
<DescriptorName UI="D000368" MajorTopicYN="N">Aged</DescriptorName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D000914" MajorTopicYN="N">Antibodies, Viral</DescriptorName>
<QualifierName UI="Q000097" MajorTopicYN="N">blood</QualifierName>
<QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D000073640" MajorTopicYN="N">Betacoronavirus</DescriptorName>
<QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName>
<QualifierName UI="Q000472" MajorTopicYN="N">pathogenicity</QualifierName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D018352" MajorTopicYN="N">Coronavirus Infections</DescriptorName>
<QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName>
<QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D006115" MajorTopicYN="N" Type="Geographic">Greece</DescriptorName>
<QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D007074" MajorTopicYN="N">Immunoglobulin G</DescriptorName>
<QualifierName UI="Q000097" MajorTopicYN="N">blood</QualifierName>
<QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D008875" MajorTopicYN="N">Middle Aged</DescriptorName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D058873" MajorTopicYN="Y">Pandemics</DescriptorName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D011024" MajorTopicYN="N">Pneumonia, Viral</DescriptorName>
<QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName>
<QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D019095" MajorTopicYN="N">Point-of-Care Systems</DescriptorName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName>
</MeshHeading>
</MeshHeadingList>
<KeywordList Owner="NOTNLM">
<Keyword MajorTopicYN="N">COVID-19</Keyword>
<Keyword MajorTopicYN="N">IgG</Keyword>
<Keyword MajorTopicYN="N">IgM</Keyword>
<Keyword MajorTopicYN="N">SARS-CoV-2</Keyword>
<Keyword MajorTopicYN="N">antibodies</Keyword>
<Keyword MajorTopicYN="N">diagnostics</Keyword>
<Keyword MajorTopicYN="N">novel coronavirus</Keyword>
<Keyword MajorTopicYN="N">point-of-care testing</Keyword>
</KeywordList>
</MedlineCitation>
<PubmedData>
<History>
<PubMedPubDate PubStatus="received">
<Year>2020</Year>
<Month>06</Month>
<Day>02</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="revised">
<Year>2020</Year>
<Month>06</Month>
<Day>15</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted">
<Year>2020</Year>
<Month>06</Month>
<Day>16</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez">
<Year>2020</Year>
<Month>9</Month>
<Day>3</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed">
<Year>2020</Year>
<Month>9</Month>
<Day>3</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline">
<Year>2020</Year>
<Month>9</Month>
<Day>8</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList>
<ArticleId IdType="pubmed">32871850</ArticleId>
<ArticleId IdType="pii">34/5/3039</ArticleId>
<ArticleId IdType="doi">10.21873/invivo.12138</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
<affiliations>
<list>
<country>
<li>Grèce</li>
</country>
<region>
<li>Attique (région)</li>
</region>
<settlement>
<li>Athènes</li>
</settlement>
</list>
<tree>
<country name="Grèce">
<region name="Attique (région)">
<name sortKey="Fragkou, Paraskevi C" sort="Fragkou, Paraskevi C" uniqKey="Fragkou P" first="Paraskevi C" last="Fragkou">Paraskevi C. Fragkou</name>
</region>
<name sortKey="Antoniadou, Anastasia" sort="Antoniadou, Anastasia" uniqKey="Antoniadou A" first="Anastasia" last="Antoniadou">Anastasia Antoniadou</name>
<name sortKey="Kavvatha, Dimitra" sort="Kavvatha, Dimitra" uniqKey="Kavvatha D" first="Dimitra" last="Kavvatha">Dimitra Kavvatha</name>
<name sortKey="Kelekis, Alexis" sort="Kelekis, Alexis" uniqKey="Kelekis A" first="Alexis" last="Kelekis">Alexis Kelekis</name>
<name sortKey="Pantazis, Nikos" sort="Pantazis, Nikos" uniqKey="Pantazis N" first="Nikos" last="Pantazis">Nikos Pantazis</name>
<name sortKey="Papaevangelou, Vassiliki" sort="Papaevangelou, Vassiliki" uniqKey="Papaevangelou V" first="Vassiliki" last="Papaevangelou">Vassiliki Papaevangelou</name>
<name sortKey="Ploussi, Agapi" sort="Ploussi, Agapi" uniqKey="Ploussi A" first="Agapi" last="Ploussi">Agapi Ploussi</name>
<name sortKey="Pournaras, Spyridon A" sort="Pournaras, Spyridon A" uniqKey="Pournaras S" first="Spyridon A" last="Pournaras">Spyridon A. Pournaras</name>
<name sortKey="Psarrakis, Christos" sort="Psarrakis, Christos" uniqKey="Psarrakis C" first="Christos" last="Psarrakis">Christos Psarrakis</name>
<name sortKey="Sirmpilantze, Tamta" sort="Sirmpilantze, Tamta" uniqKey="Sirmpilantze T" first="Tamta" last="Sirmpilantze">Tamta Sirmpilantze</name>
<name sortKey="Tsiodras, Sotirios" sort="Tsiodras, Sotirios" uniqKey="Tsiodras S" first="Sotirios" last="Tsiodras">Sotirios Tsiodras</name>
</country>
</tree>
</affiliations>
</record>

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